Global DNA hypomethylation in breast carcinoma: correlation with prognostic factors and tumor progression.

摘要: BACKGROUND. The global DNA methylation of 136 breast lesions (117 primary invasive carcinomas, 5 benign phyllodes tumors, 11 fibroadenomas, and 3 sclerosing adenosis) their respective adjacent parenchyma was analyzed using an in vitro enzyme assay. METHODS. In the group patients with carcinoma, hypomethylation correlated clinical pathologic parameters known to affect disease prognosis. Histopathologic type, stage, tumor grade were evaluated according World Health Organization classification, TNM system, criteria Elston Ellis’ criteria, respectively. flow cytometry performed fresh/frozen samples stained propidium iodide. Hormone receptor (estrogen progesterone receptor) status determined by immunocytochemistry. RESULTS. comparative study showed that carcinomas statistically significantly less methylated than (P 0.0001), 0.0002), normal , 0.0001). A significant correlation found between stage 0.0009), size 0.0026), histologic 0.0097) malignant neoplasms. trend for from positive axillary lymph nodes (N1) be more hypomethylated those without nodal involvement (N0) 0.055) verified. contrast, no association type hormone receptors, ploidy, S-phase fraction. CONCLUSIONS. current shows is increased playing a potentially important role development. These findings also suggest may biologic marker prognostic significance this Cancer 1999;85:112‐ 8. © 1999 American Society.