The Role of Antigenic Determinants in the Control of IgM and IgG Antibody Responses to Denatured DNA

摘要: Rabbits immunized with complexes of denatured calf thymus DNA and methylated bovine serum albumin (MBSA) produced only IgM antibodies to DNA, confirming the results previous studies. In contrast, both IgG were induced by MBSA T4 T6 bacteriophage in which normal base cytosine is replaced glucosylated hydroxymethylcytosine. The glucose not essential for this switch, since a preparation modified T2 phage contained nonglucosylated hydroxymethylcytosine, also formation classes antibodies. With or without glycosylation, hydroxymethylcytosine served as dominating determinant specificity well signal 7S antibody production. validity complement fixation an assay these responses was confirmed quantitative precipitation tests.