Controlled release of BMP-2 by chitosan/γ-PGA polyelectrolyte multilayers coating on titanium alloy promotes osteogenic differentiation in rat bone-marrow mesenchymal stem cells
作者： Ren-Jei Chung , Keng-Liang Ou , Wei-Kai Tseng , Hsuan-Liang Liu
DOI: 10.1016/J.SURFCOAT.2016.03.081
关键词:
摘要: Abstract The excellent biocompatibility and mechanical properties of titanium alloys have led to their wide-scale application in orthopedic implants. Appropriate surface treatment these can greatly improve cellular attachment as well interactions between implants tissue. This paper reports on the preparation polyelectrolyte multilayers (PEM) coating comprising chitosan gamma-poly(glutamic acid) a Ti6Al4V substrate via spin-coating. Bone morphogenetic protein 2 was then loaded directly into coating. Following sample preparation, we performed vitro studies using SD rat bone-marrow mesenchymal stem cells (rBMSCs) investigate biological effects Our results demonstrate that this chitosan/γ-PGA PEM is highly stable does not any adverse rBMSCs. proposed proved effective carrying enabling gradual release BMP-2. At day 14, coated samples presented osteogenic differentiation far exceeding non-coated samples. After co-culturing for 21 days, calcium content group double group, which clear indication enhanced mineralization.
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