Energy requirement for specific transcription initiation by the human RNA polymerase II system.

摘要: The energy requirement for specific transcription initiation and elongation by the human RNA polymerase II system was studied in vitro using partially purified factors from HeLa cell nuclear extracts. synthesis of 536-nucleotide long run-off transcript resulting at adenovirus major late promoter found to be dependent upon presence either ATP or dATP (with imido derivative adenyl-5‘-yl imidodiphosphate being used as substrate reaction). An identical hydrolysis phosphate bond an adenosine nucleotide observed decanucleotide transcribed absence GTP substrate. In contrast, nonhydrolyzable analog fully substitutes during subsequent these short transcripts, which demonstrates that occurs earlier step reaction. Thus particular factor requires (or dATP) its function must act prior to, concomitant with, formation first few phosphodiester linkages II.