Transcriptional control regions: nucleotide sequence requirements for initiation by RNA polymerase II and III.

摘要: Until recently, the nucleotide sequences involved in initiation of transcription within eukaryotic cells remained a mystery. Now, thanks to variety technological advances, it is possible identify these and appreciate their functions process. Cloning technology enables one isolate study genes individually. Rapid DNA RNA sequencing procedures facilitate sequence analysis both RNAs they encode. A vitro mutagenesis protocols makes direct deletions single base-pair changes specific sequences. Selection are available return which have been manipulated for analysis. And, finally, systems developed accurately transcribe either polymerase II- or Ill-type genes. Genes can now be isolated, sequenced, mutated, studied vivo vitro. As result, process related