Transcriptional regulation of genes involved in Zn transport after foliar Zn application to Medicago sativa

摘要: ABSTRACT Zinc (Zn) is an essential micronutrient for both plants and animals, Zn deficiency one of the most widespread problems agricultural production. Although many studies have been performed on biofortification staple crops with Zn, few focused forage crops. In this study molecular mechanisms transport-related in Medicago sativa L. were investigated following foliar applications aimed at increasing accumulation edible tissues. uptake redistribution between shoot root determined application six doses to leaves (0, 0.01, 0.1, 0.5, 1, 10 mg plant-1). Twelve putative genes encoding proteins involved transport (MsZIP1-7, MsZIF1, MsMTP1, MsYSL1, MsHMA4 MsNAS1) identified changes their expression quantified using newly designed RT-qPCR assays. Shoot concentration was increased ≥ 0.1 plant-1. Increased MsZIP2, MsNAS1 shoots, MsZIP2 roots, observed largest dose. By contrast, MsZIP3 downregulated shoots Three functional modules M. response application: by cells, vacuolar sequestration within plant. These results will inform genetic engineering strategies efficiency crop biofortification. One-sentence summary Upregulation ZIP2, NASI HMA4 downregulation ZIP3 are associated shoot-to-root translocation