Pyruvate stimulates mitophagy via PINK1 stabilization

摘要: Damaged mitochondria are targeted for degradation by an autophagy pathway known as mitophagy. Despite efforts to unravel the mechanisms underlying mitophagy, aspects of mitophagy regulation remain largely unknown. In this study, using a cell-based fluorescence assay reflecting CCCP-induced we have screened cDNA expression library encoding mitochondrial proteins and identified PDK4 regulator. Ectopic stimulated clearance during enhanced pyruvate levels in both cytosol mitochondria. Interestingly, was not efficient absence pyruvate. Pyruvate required PINK1 stabilization depolarization subsequent PARK2 translocation LC3 recruitment onto damaged This pyruvate-mediated affected OXPHOS or cellular ATP levels, thus independent energy metabolism. Rather, interaction between TOMM20 under CCCP condition. These results suggest that is PINK1/PARK2-mediated