Physical state of human papillomavirus type 16 in cervical intraepithelial lesions and cancers determined by two different quantitative real-time PCR methods
作者： Slawa Szostek , Beata Biesaga , Barbara Zawilinska , Malgorzata Klimek , Magdalena Kosz-Vnenchak
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摘要: The aim of this study was to analyse the correlation between a new multiplex qPCR assay and reference for assessment human papillomavirus (HPV16) load viral genome status. performed on 100 HPV16 positive samples containing premalignant lesions carcinomas. E2 E6 gene loads were assessed by two PCR methods. normalized cell number targeting RNase P open reading frame. physical state determined as ratio E2/E6 copies per cell. Among analysed, there no statistically significant differences in evaluated qPCR, coefficients 0.98 0.97, respectively. There 19% with integrated, 73% mixed 8% episomal detected 17%, 79%, 4%, respectively, found qPCR. Prevalence integrated forms estimated higher than one obtained (Chi2, p < 0.0001), but malignant diagnoses demonstrated regardless methods used. Sensitivity specificity 93.7% 100% compared predictive value 100%. In summary, respect frequency status shown be sensitive specific method. Simultaneous estimation genes reaction tube reduces cost testing.
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