Generation of Temperature-Sensitive Cbp1 Strains of Saccharomyces Cerevisiae by Pcr Mutagenesis and in Vivo Recombination: Characteristics of the Mutant Strains Imply That Cbp1 Is Involved in Stabilization and Processing of Cytochrome B Pre-mRNA

摘要: Mitochondrial biogenesis is dependent on both nuclearly and mitochondrially encoded proteins. Study of the mitochondrial gene products their effect genome expression essential to understanding function. Mutations in nuclear CBP1 Saccharomyces cerevisiae result degradation cytochrome b (cob) RNA; thus, cells are unable respire. Putative roles for protein include processing precursor RNA yield mature 5' end cob mRNA and/or physical protection from by nucleases. To examine activity CBP1, we generated temperature-sensitive cbp1 mutant strains polymerase chain reaction (PCR) mutagenesis vivo recombination. These lack only at nonpermissive temperature. Quantitative primer extension analyses these a deletion strain demonstrated that required stability RNAs addition production stable mRNA. Thus, not involved solely Moreover, found mRNAs undetectable while reduced slightly Collectively, data lead us favor hypothesis whereby protects promotes event generates