Measurement and analysis of yeast pre-mRNA sequence contribution to splicing efficiency
作者： B.C. Rymond , C. Pikielny , B. Seraphin , P. Legrain , M. Rosbash
DOI: 10.1016/0076-6879(90)81116-C
关键词:
摘要: Publisher Summary This chapter examines yeast pre-mRNA sequence contribution to splicing efficiency. A primer complementary intron sequences, RB27 can be used determine the relative abundance of premRNA and lariat molecules. On addition a small, splicing-competent substrate ATP whole cell extract, time-dependent appearance three complexes could visualized. Presumably carrier RNA eliminates nonspecific adventitious binding proteins spliceosome complexes, thereby increasing electrophoretic mobility improving resolution. The modified is then added an in vitro assembly reaction, are resolved on native polyacrylamide gels. expectation that process should sensitive chemical modification at nucleotide positions required for formation spliceosomes. If particular extremely deleterious it absent from complex-derived RNA. DNA oligonucleotides size-selected by HPLC chromatography or gel electrophoresis prior labeling. precaution removes shorter, incompletely synthesized molecules compete labeling may lead background problems during extension.
sci-hub.se 参考文章(40)
Richard W. Shulman, Jonathan R. Warner, Ribosomal RNA transcription in a mutant of Saccharomyces cerevisiae defective in ribosomal protein synthesis. Molecular Genetics and Genomics. ,vol. 161, pp. 221- 223 ,(1978) , 10.1007/BF00274191
U. Vijayraghavan, R. Parker, J. Tamm, Y. Iimura, J. Rossi, J. Abelson, C. Guthrie, Mutations in conserved intron sequences affect multiple steps in the yeast splicing pathway, particularly assembly of the spliceosome. The EMBO Journal. ,vol. 5, pp. 1683- 1695 ,(1986) , 10.1002/J.1460-2075.1986.TB04412.X
B. Séraphin, L. Kretzner, M. Rosbash, A U1 snRNA:pre-mRNA base pairing interaction is required early in yeast spliceosome assembly but does not uniquely define the 5' cleavage site. The EMBO Journal. ,vol. 7, pp. 2533- 2538 ,(1988) , 10.1002/J.1460-2075.1988.TB03101.X
H.M. Fried, N.J. Pearson, C.H. Kim, J.R. Warner, The genes for fifteen ribosomal proteins of Saccharomyces cerevisiae. Journal of Biological Chemistry. ,vol. 256, pp. 10176- 10183 ,(1981) , 10.1016/S0021-9258(19)68759-4
R J Lin, A J Newman, S C Cheng, J Abelson, Yeast mRNA splicing in vitro. Journal of Biological Chemistry. ,vol. 260, pp. 14780- 14792 ,(1985) , 10.1016/S0021-9258(17)38641-6
John L. Teem, Jose R. Rodriguez, Lin Tung, Michael Rosbash, The rna2 mutation of yeast affects the processing of actin mRNA as well as ribosomal protein mRNAs Molecular Genetics and Genomics. ,vol. 192, pp. 101- 103 ,(1983) , 10.1007/BF00327653
Albert E. Dahlberg, C.Wesley Dingman, Andrew C. Peacock, Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels Journal of Molecular Biology. ,vol. 41, pp. 139- 147 ,(1969) , 10.1016/0022-2836(69)90131-4
Leland H. Hartwell, Calvin S. McLaughlin, Jonathan R. Warner, Identification of ten genes that control ribosome formation in yeast. Molecular Genetics and Genomics. ,vol. 109, pp. 42- 56 ,(1970) , 10.1007/BF00334045
D FRENDEWEY, W KELLER, Stepwise assembly of a pre-mRNA splicing complex requires U-snRNPs and specific intron sequences. Cell. ,vol. 42, pp. 355- 367 ,(1985) , 10.1016/S0092-8674(85)80131-8
William Hendrickson, Robert F. Schleif, Regulation of the Escherichia coli l-arabinose operon studied by gel electrophoresis DNA binding assay☆ Journal of Molecular Biology. ,vol. 178, pp. 611- 628 ,(1984) , 10.1016/0022-2836(84)90241-9