Protective effects of protein hydrolysate from marine microalgae Navicula incerta on ethanol-induced toxicity in HepG2/CYP2E1 cells
作者： Kyong-Hwa Kang , Zhong-Ji Qian , BoMi Ryu , Daekyung Kim , Se-Kwon Kim
DOI: 10.1016/J.FOODCHEM.2011.10.031
关键词:
摘要: Abstract Ethanol is independently known to cause tissue damage through various mechanisms. This study was designed evaluate the protective effect of marine microalgae, Navicula incerta protein enzymatic hydrolysates (NEHs) against ethanol-induced hepatotoxicity in HepG2 cells transfected with human CYP2E1. Induction CYP2E1 by ethanol one central pathways which generates a state oxidative stress hepatocytes. When alcohol-induced were treated NEHs at concentrations, there dose-dependent decrease gamma-glutamyl transpeptidase (GGT) activity culture media and loss cell viability. Among constituents obtained papain (P-NEH), pronase-E (PR-NEH) α-chymotrypsin (A-NEH) attenuated cytotoxicity effectively, respectively. The appeared be GGT inhibitor. Therefore, cytoprotective effects HepG2/CYP2E1 could attributed inhibition NEHs. suggests that have enough potential considered as highly active compounds toxicity leads significant nutraceuticals.
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sci-hub.se 参考文章(37)
Mary E. Anderson, [70] Determination of glutathione and glutathione disulfide in biological samples Glutamate, Glutamine, Glutathione, and Related Compounds. ,vol. 113, pp. 548- 555 ,(1985) , 10.1016/S0076-6879(85)13073-9
Hongqiao Zhang, Henry Jay Forman, Jinah Choi, γ‐Glutamyl Transpeptidase in Glutathione Biosynthesis Methods in Enzymology. ,vol. 401, pp. 468- 483 ,(2005) , 10.1016/S0076-6879(05)01028-1
François Denizot, Rita Lang, Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Journal of Immunological Methods. ,vol. 89, pp. 271- 277 ,(1986) , 10.1016/0022-1759(86)90368-6
C J Glueck, E Hogg, C Allen, P S Gartside, Effects of alcohol ingestion on lipids and lipoproteins in normal men: isocaloric metabolic studies. The American Journal of Clinical Nutrition. ,vol. 33, pp. 2287- 2293 ,(1980) , 10.1093/AJCN/33.11.2287
Peter J. Reeds, Peter J. Garlick, Protein and Amino Acid Requirements and the Composition of Complementary Foods Journal of Nutrition. ,vol. 133, ,(2003) , 10.1093/JN/133.9.2953S
P.J.M.J. Kok, B. Seidel, H.C. Holtkamp, J. Huisman, A new procedure for the visualization of multiple forms of gamma-glutamyl transferase (GGT) results in normals, patients receiving enzyme-inducing drugs and patients having liver parenchymal lesions Clinica Chimica Acta. ,vol. 90, pp. 209- 216 ,(1978) , 10.1016/0009-8981(78)90259-0
Paul M. Silber, A.Jay Gandolfi, Klaus Brendel, Adaptation of a γ-glutamyl transpeptidase assay to microtiter plates Analytical Biochemistry. ,vol. 158, pp. 68- 71 ,(1986) , 10.1016/0003-2697(86)90590-7
Nancy G. Misslbeck, T.Colin Campbell, Daphne A. Roe, Increase in hepatic gamma-glutamyltransferase (GGT) activity following chronic ethanol intake in combination with a high fat diet☆☆☆ Biochemical Pharmacology. ,vol. 35, pp. 399- 404 ,(1986) , 10.1016/0006-2952(86)90211-X
Masahiro Tohkin, Hiroshi Hikino, Masao Hattori, Tatsuya Sakamoto, Tsuneo Namba, Yoshinobu Kiso, Mechanism of antihepatotoxic activity of glycyrrhizin. I: Effect on free radical generation and lipid peroxidation. Planta Medica. ,vol. 50, pp. 298- 302 ,(1984) , 10.1055/S-2007-969714
Arthur I Cederbaum, Defeng Wu, Montserrat Mari, Jingxiang Bai, CYP2E1-dependent toxicity and oxidative stress in HepG2 cells, Free Radical Biology and Medicine. ,vol. 31, pp. 1539- 1543 ,(2001) , 10.1016/S0891-5849(01)00743-2