Mixed micelles based on a pH-sensitive prodrug and TPGS for enhancing drug efficacy against multidrug-resistant cancer cells.
作者： Dandan Sun , Xueming Lv , Xinnan Wang , Ao Yu , Yongjian Wang
DOI: 10.1016/J.COLSURFB.2017.07.082
关键词:
摘要: Abstract In this study, we prepared mixed micelles composed of a pH-sensitive poly(ethylene glycol)-doxorubicin conjugate prodrug and d-alpha-tocopheryl polyethylene glycol succinate (TPGS). The average hydrodynamic size the was approximately 144 nm, measured by dynamic light scattering. an MTT assay non-cytotoxic at low concentration but inhibited drug-resistant cancer cell (MCF-7/ADR) growth high dose. showed concentration-dependent cytotoxicity significantly increased in MCF-7/ADR cells. Confocal laser scanning images revealed that higher concentrations doxorubicin were successfully delivered into nuclei, enabling effective drug-induced death. Fluorescence microscopy indicated there less escape internalized from Therefore, enhanced drug efficacy cells is most likely attributed to synergistic effect drug-release inside suppression P-glycoprotein efflux activity TPGS.
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