Induced pluripotent stem cells: opportunities and challenges.
作者： Keisuke Okita , Shinya Yamanaka
关键词:
摘要: Somatic cells have been reprogrammed into pluripotent stem by introducing a combination of several transcription factors, such as Oct3/4, Sox2, Klf4 and c-Myc. Induced (iPS) from patient's somatic could be useful source for drug discovery cell transplantation therapies. However, most human iPS are made viral vectors, retrovirus lentivirus, which integrate the reprogramming factors host genomes may increase risk tumour formation. Several non-integration methods reported to overcome safety concern associated with generation cells, transient expression using adenovirus vectors or plasmids, direct delivery proteins. Although these avoid genomic alteration they inefficient. studies gene expression, epigenetic modification differentiation revealed insufficient thus suggesting need improvement procedure not only in quantity but also quality. This report will summarize current knowledge discuss future medical application.
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