Embryo and endosperm development is disrupted in the female gametophytic capulet mutants of Arabidopsis.

摘要: The female gametophyte of higher plants gives rise, by double fertilization, to the diploid embryo and triploid endosperm, which develop in concert produce mature seed. What roles gametophytic maternal factors play this process is not clear. female-gametophytic effects on endosperm development Arabidopsis mea, fis, fie mutants appear be due gametic imprinting that can suppressed METHYL TRANSFERASE1 antisense (MET1 a/s) transgene expression or mutation DECREASE IN DNA METHYLATION1 (DDM1) gene. Here we describe two novel maternal-effect mutants, capulet1 (cap1) capulet2 (cap2). In cap1 mutant, both are arrested at early stages. cap2 blocked very stages, whereas embryos heart stage. cap mutant phenotypes were rescued wild-type pollen nor from tetraploid plants. Furthermore, removal silencing barriers paternal genome MET1 a/s ddm1 also failed restore seed mutants. Neither displayed autonomous development, contrast addition, was epistatic fis1 sexual development. Finally, endosperms, like expressed paternally inactive endosperm-specific FIS2 promoter GUS fusion only when introduced via sac, indicating affected. Our results suggest CAP genes represent functions supplied required for