CARMIL3 is important for cell migration and morphogenesis during early development in zebrafish

摘要: Cell migration is important during early animal embryogenesis. and cell shape are controlled by actin assembly dynamics, which depend on capping proteins, including the barbed-end heterodimeric protein (CP). CP activity can be regulated capping-protein-interacting (CPI) motif CARMIL (capping Arp2/3 myosin-I linker) family proteins. Previous studies of CARMIL3, one three highly conserved genes in vertebrates, have largely been limited to cells culture. Towards understanding function embryogenesis vivo, we analyzed zebrafish lines carrying mutations carmil3. Maternal-zygotic mutants show impaired endodermal gastrulation, along with defects dorsal forerunner (DFC) cluster formation, affecting morphogenesis Kupffers vesicle (KV). Mutant KVs smaller display decreased numbers cilia, leading left/right (L/R) patterning variable penetrance expressivity. The expressivity KV phenotype carmil3 correlated well L/R heart positioning defect at end This first vivo study CARMIL3 reveals its new role for vertebrate embryo. involves DFCs, subsequent asymmetry.