Generation of mice with a conditional allele of the p120 Ras GTPase-activating protein.

摘要: p120 Ras GTPase-activating protein (RasGAP) encoded by the rasa1 gene in mice is a prototypical member of RasGAP family proteins involved negative-regulation p21 proto-oncogene. has been implicated signal transduction through number cell surface receptors. In humans, inactivating mutations coding region RASA1 cause capillary malformation arteriovenous malformation. mice, generalized disruption results early embryonic lethality associated with defective vasculogenesis and increased apoptosis neuronal cells. The this mouse model precludes its use to further study importance as regulator function. Therefore, circumvent problem, we have generated conditional knockout mouse. mouse, an exon that encodes part essential for catalytic activity flanked loxP recognition sites. With different constitutive inducible Cre transgenic lines, show deletion from locus effective loss expression catalytically-active variety adult tissues. will be useful analysis role mature types.