Survival of rabbit limbal stem cell allografts.

摘要: Failure of a specialized population corneal epithelial stem cells found in the peripheral cornea and limbus results ocular surface disease, which may be amenable to treatment by transplantation limbal tissue. This study was designed investigate donor cell allograft survival rabbits with disease. Rabbits underwent debridement ablation induce disease were then treated allotransplantation, allotransplantation plus topical steroid, or steroid only (n = 7 for each group). Donors recipients sex mismatched. Recipients followed up 5 months. Outcome assessed daily slit-lamp examination, weekly impression cytology photographic record, end-point chromatin fluorescent tracer analyses, histology, immunohistochemistry. In no case completely normal regained, but some animals that received grafts corticosteroids fared best all criteria used. absence immunosuppression, graft hemorrhagia (believed manifestation rejection) occurred within first month, became resurfaced conjunctiva-derived cells, survived centrally long term. Topical reduced number severity these episodes significantly, associated donor-derived central grafted animals. Thus, rabbit allografts appeared undergo rejection, could modified useful regeneration where rejection circumvented.