EFFECT OF AUTOCLAVING ON PROTEIN CONTENT USING SDS- PAGE

作者: Nitin Puranik , Pramod Kumar Singh , Vidhi Tiwari , Neha Panwar

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摘要: Pulses have high value of nutritional properties but underutilized due to the presence some toxic substances reported as anti-nutritional factors. Different homemade processing methods (germination, boiling, and pressure cooking roasting) were employed reduce these factors in pulses. Among various treatments employed; resulted maximum reduction literature. Being valuable it also adversely affects protein contents In this study we evaluated effect on profile pulses such albumin, globulin total proteins. We found albumin globulins proteins contents.

参考文章(10)
S.S. Jha, D. Ohri, Comparative Study of Seed Protein Profiles in the Genus Pisum Biologia plantarum. ,vol. 45, pp. 529- 532 ,(2002) , 10.1023/A:1022316907401
M. Takaki, C. C. Custodio, N. B. Machado, Evaluation of naturally and artificially aged seeds of Phaseolus vulgaris L. Seed Science and Technology. ,vol. 29, pp. 137- 149 ,(2001)
Sameer. S. Bhagyawant, Nidhi Shrivastava, Pramod Kumar Singh, Bechan Sharma, Effect of Domestic Processes on Chickpea Seeds for Antinutritional Contents and Their Divergence American Journal of Food Science and Technology. ,vol. 3, pp. 111- 117 ,(2015) , 10.12691/AJFST-3-4-3
Ulrich K. Laemmli, Rodney A. Johnson, Maturation of the head of bacteriophage T4. II. Head-related, aberrant tau-particles. Journal of Molecular Biology. ,vol. 80, pp. 601- 611 ,(1973) , 10.1016/0022-2836(73)90199-X
Anne Pihlanto, Hannu Korhonen, Bioactive peptides and proteins. Advances in food and nutrition research. ,vol. 47, pp. 175- 276 ,(2003) , 10.1016/S1043-4526(03)47004-6
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
R Munirajappa, K T Pandurange Gowda, K Bhanuprakash, B N Radha, B K Ramachandrappa, B C Channakeshava, Nagaraj Hullur, Effect of seed ageing on protein quality and quantity in maize International Journal of Bioassays. ,vol. 3, pp. 1708- 1713 ,(2014) , 10.21746/IJBIO.2014.01.0014
Yves D. Sauvaire, Jean Claude F. Baccou, Karoly Kobrehel, Solubilization and characterization of fenugreek seed proteins. Journal of Agricultural and Food Chemistry. ,vol. 32, pp. 41- 47 ,(1984) , 10.1021/JF00121A011
U.K. Laemmli, M. Favre, Maturation of the head of bacteriophage T4 Journal of Molecular Biology. ,vol. 80, pp. 575- 599 ,(1973) , 10.1016/0022-2836(73)90198-8