Dairy cows produce cytokine and cytotoxic T cell responses following vaccination with an antigenic fraction from Streptococcus uberis.

摘要: Abstract Streptococcus uberis is a major cause of mastitis in dairy cows worldwide and currently, there no vaccine commercially available against this form mastitis. In the current study, cell-free extracts (CFE) were prepared from each three different S. strains, designated as #3, #24 #363 representative main sequence types that New Zealand. These proteins formulated into vaccines with Emulsigen-D immunogenicity was determined both calves cows. Two groups ( n  = 5/group) vaccinated subcutaneously CFE strain or strains Emulsigen-D, respectively. A third group  = 5) also containing recombinant bovine granulocyte macrophage colony-stimulating factor while, control not vaccinated. Vaccinated animals produced strong antibody responses to antigens an antigen-specific cytotoxic effect blood monocytes/macrophages had phagocytosed , significant differences observed between groups. second trial, safety all six at 3 1 week prior dry off revaccinated 2–3 weeks before calving. Immune mammary gland secretions (MGS) monitored during period subsequent lactation. The well tolerated adverse vaccination any Vaccination induced moderate IFN-γ MGS. conclusion, results suggest cattle by subcutaneous route can induce cellular humoral responses.