Multiplex DNA assay based on nanoparticle probes by single particle inductively coupled plasma mass spectrometry.
作者： Shixi Zhang , Guojun Han , Zhi Xing , Sichun Zhang , Xinrong Zhang
DOI: 10.1021/AC404245Z
关键词:
摘要: A multiplex DNA assay based on nanoparticle (NP) tags detection utilizing single particle mode inductively coupled plasma mass spectrometry (SP-ICP-MS) as ultrasensitive readout has been demonstrated in the article. Three targets associated with clinical diseases (HIV, HAV, and HBV) down to 1 pM were detected by probes labeled AuNPs, AgNPs, PtNPs via sandwich assay. Single nucleotide polymorphisms genes can also be effectively discriminated. Since our method is unaffected sample matrix, it well-suited for diagnostic applications. Moreover, high sensitivity of SP-ICP-MS variety NPs detectable SP-ICP-MS, high-throughput could achieved without signal amplification or chain reaction amplification.
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sci-hub.se 参考文章(33)
Yougen Li, Yen Thi Hong Cu, Dan Luo, None, Multiplexed detection of pathogen DNA with DNA-based fluorescence nanobarcodes Nature Biotechnology. ,vol. 23, pp. 885- 889 ,(2005) , 10.1038/NBT1106
R. Allabashi, W. Stach, A. de la Escosura-Muñiz, L. Liste-Calleja, A. Merkoçi, ICP-MS: a powerful technique for quantitative determination of gold nanoparticles without previous dissolving Journal of Nanoparticle Research. ,vol. 11, pp. 2003- 2011 ,(2009) , 10.1007/S11051-008-9561-2
Robert Elghanian, James J. Storhoff, Robert C. Mucic, Robert L. Letsinger, Chad A. Mirkin, Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles Science. ,vol. 277, pp. 1078- 1081 ,(1997) , 10.1126/SCIENCE.277.5329.1078
Joseph Wang, Guodong Liu, Arben Merkoçi, Electrochemical coding technology for simultaneous detection of multiple DNA targets. Journal of the American Chemical Society. ,vol. 125, pp. 3214- 3215 ,(2003) , 10.1021/JA029668Z
W. Russ Algar, Ulrich J. Krull, Toward A Multiplexed Solid-Phase Nucleic Acid Hybridization Assay Using Quantum Dots as Donors in Fluorescence Resonance Energy Transfer Analytical Chemistry. ,vol. 81, pp. 4113- 4120 ,(2009) , 10.1021/AC900421P
Xiaoqing Liu, Fuan Wang, Ruth Aizen, Omer Yehezkeli, Itamar Willner, Graphene Oxide/Nucleic-Acid-Stabilized Silver Nanoclusters: Functional Hybrid Materials for Optical Aptamer Sensing and Multiplexed Analysis of Pathogenic DNAs Journal of the American Chemical Society. ,vol. 135, pp. 11832- 11839 ,(2013) , 10.1021/JA403485R
Rahela Gasparac, Bradford J. Taft, Melissa A. Lapierre-Devlin, Adam D. Lazareck, Jimmy M. Xu, Shana O. Kelley, Ultrasensitive Electrocatalytic DNA Detection at Two- and Three-Dimensional Nanoelectrodes Journal of the American Chemical Society. ,vol. 126, pp. 12270- 12271 ,(2004) , 10.1021/JA0458221
Francis S. Collins, , Eric D. Green, Alan E. Guttmacher, Mark S. Guyer, A vision for the future of genomics research Nature. ,vol. 422, pp. 835- 847 ,(2003) , 10.1038/NATURE01626
W. Russ Algar, Ulrich J. Krull, Multiplexed Interfacial Transduction of Nucleic Acid Hybridization Using a Single Color of Immobilized Quantum Dot Donor and Two Acceptors in Fluorescence Resonance Energy Transfer Analytical Chemistry. ,vol. 82, pp. 400- 405 ,(2010) , 10.1021/AC902221D
C. Degueldre, P.-Y. Favarger, R. Rossé, S. Wold, Uranium colloid analysis by single particle inductively coupled plasma-mass spectrometry Talanta. ,vol. 68, pp. 623- 628 ,(2004) , 10.1016/J.TALANTA.2005.05.006