A facile fluorescence method for versatile biomolecular detection based on pristine α-Fe2O3 nanoparticle-induced fluorescence quenching

摘要: Abstract This work investigated the interactions of α-Fe 2 O 3 nanoparticles (NPs) with different structural nucleic acids and their fluorescence quenching ability towards fluorophore-labelled acid probes. Different from bulk samples, nanoscale particles exhibit unique properties strong adsorption to single-stranded DNA (ssDNA) Based on these findings, a facile method was developed for versatile quantification acids. The size scale NPs makes significant impact this sensing platform. Better selectivity given by bigger NP (50–100 nm)-based acid-sensing platform compared smaller (30 nm)-based one. In 50–100 nm NP-based platform, single nucleotide mismatch or base-pair can even be effectively discriminated. targets micro-RNA (miRNA), ssDNA double-stranded (dsDNA) are sensitively detected detection limits 0.8 nM, 1.1 nM 0.64 nM ( S / N =3), respectively. Significantly, possess affinities probes lengths, used as universal quencher labelled fluorescent dyes. On basis properties, pristine hold potential widely utilized in development novel biosensors signal amplification simultaneous multiple target strategies.