Identification of new biomarkers in immune microenvironment of testicular germ cell tumour
作者： Xiaoqiang Liu , Yuxuan Song , Xiao Wang , Jun Zhu , Ningjing Ou
DOI: 10.1111/AND.13986
关键词:
摘要: To seek novel prognostic biomarkers for testicular germ cell tumour (TGCT) and investigate the immune microenvironment, we identified critical differentially expressed genes (DEGs) by overlapping GSE1818 dataset from Gene Expression Omnibus (GEO). Protein-protein interaction (PPI) network was used to key modules hub genes. Functional enrichment analysis performed underlying molecular functions of DEGs in TGCT development progression. The following survival based on Cancer Genome Atlas (TCGA) indicated that AKAP4, SPA17 TNP1 are correlated with prognosis. Immunohistochemistry quantitative real-time polymerase chain reaction verified down-regulation 3 TGCT. set conducted further explore role respectively. In addition, samples had high infiltration CD8+ T cells, M0 M1 macrophage resting myeloid dendritic cells microenvironment. We also constructed microRNA-gene regulatory networks identify upstream microRNAs conclusion, our findings promising AKAP4 might regulate
sci-hub.st 参考文章(51)
, Prognostic Factors in Patients With Metastatic Germ Cell Tumors Who Experienced Treatment Failure With Cisplatin-Based First-Line Chemotherapy Journal of Clinical Oncology. ,vol. 28, pp. 4906- 4911 ,(2010) , 10.1200/JCO.2009.26.8128
Yang Liu, Xin Tian, Shenyi Jiang, Xuemei Ren, Fengjie Liu, Jichun Yang, Yanling Chen, Youhong Jiang, Umbilical cord blood-derived dendritic cells infected by adenovirus for SP17 expression induce antigen-specific cytotoxic T cells against NSCLC cells. Cellular Immunology. ,vol. 298, pp. 18- 24 ,(2015) , 10.1016/J.CELLIMM.2015.08.004
Lanlan Hui, Ye Chen, Tumor microenvironment: Sanctuary of the devil Cancer Letters. ,vol. 368, pp. 7- 13 ,(2015) , 10.1016/J.CANLET.2015.07.039
Aaron M Newman, Chih Long Liu, Michael R Green, Andrew J Gentles, Weiguo Feng, Yue Xu, Chuong D Hoang, Maximilian Diehn, Ash A Alizadeh, Robust enumeration of cell subsets from tissue expression profiles Nature Methods. ,vol. 12, pp. 453- 457 ,(2015) , 10.1038/NMETH.3337
Monika Fijak, Andreas Meinhardt, The testis in immune privilege Immunological Reviews. ,vol. 213, pp. 66- 81 ,(2006) , 10.1111/J.1600-065X.2006.00438.X
O. Hofmann, O. L. Caballero, B. J. Stevenson, Y.-T. Chen, T. Cohen, R. Chua, C. A. Maher, S. Panji, U. Schaefer, A. Kruger, M. Lehvaslaiho, P. Carninci, Y. Hayashizaki, C. V. Jongeneel, A. J. G. Simpson, L. J. Old, W. Hide, Genome-wide analysis of cancer/testis gene expression Proceedings of the National Academy of Sciences of the United States of America. ,vol. 105, pp. 20422- 20427 ,(2008) , 10.1073/PNAS.0810777105
Tine Hvarness, John E. Nielsen, Kristian Almstrup, Niels E. Skakkebaek, Ewa Rajpert-De Meyts, Mogens H. Claesson, Phenotypic characterisation of immune cell infiltrates in testicular germ cell neoplasia. Journal of Reproductive Immunology. ,vol. 100, pp. 135- 145 ,(2013) , 10.1016/J.JRI.2013.10.005
Alisher R. Dadabayev, Zhiqing Wang, Yana Zhang, Jian Zhang, William R. Robinson, Seah H. Lim, Cancer immunotherapy targeting Sp17: When should the laboratory findings be translated to the clinics? American Journal of Hematology. ,vol. 80, pp. 6- 11 ,(2005) , 10.1002/AJH.20415
Daniela F Quail, Johanna A Joyce, Microenvironmental regulation of tumor progression and metastasis. Nature Medicine. ,vol. 19, pp. 1423- 1437 ,(2013) , 10.1038/NM.3394
Lidia C. Boffa, Vincent G. Allfrey, Changes in chromosomal proteins in colon cancer. The complexity and DNA‐binding properties of tumor‐associated proteins and evidence for their association with the malignant state in human colonic epithelium Cancer. ,vol. 40, pp. 2584- 2591 ,(1977) , 10.1002/1097-0142(197711)40:5+<2584::AID-CNCR2820400930>3.0.CO;2-C