Murine IgA binding factors produced by Fc alpha R(+) T cells: role of Fc gamma R(+) cells for the induction of Fc alpha R and formation of IgA-binding factor in Con A-activated cells.

摘要: The relationship between the production of a T cell factor having affinity for IgA (IgA-binding factor(s); BF) and expression Fc receptors specific (Fc alpha R) was studied by using murine spleen cells activated with concanavalin A (Con blasts). R detected cytophilic binding anti-TNP myeloma protein (MOPC 315 IgA) to Con blasts as determined an indirect rosette method trinitrophenylated sheep red blood (TNP-SRBC). After 18 hr preculture IgA, expressed on 15 20% blasts, which released BF suppressing in vitro synthesis stimulated pokeweed mitogen (PWM). Without there neither induction nor from blasts. not induced if gamma R(+) were depleted rosetting SRBC sensitized rabbit IgG antibody (EA gamma). Even after suppressive undetectable culture supernatant population. By double EA quail cells, proved be co-expressed precursor results suggested that both could is case T2D4 R(+), hybridoma are known produce IgG-binding factor(s) (IgG BF. single monoclonal anti-Fc (2. 4G2 ). reactivity 2. inhibition formation or cells. addition antibody, however, did affect IgA. Furthermore, already L5178Y lymphoma line inhibited antibody. confirmed distinct same their may R.