Lysophosphatidic acid (LPA) 18:1 transcriptional regulation of primary human gingival fibroblasts.

摘要: The pleiotropic, bioactive lipid lysophosphatidic acid [(LPA), 1-acyl-sn-glycerol-3-phosphate] exerts critical regulatory actions in physiology and pathophysiology many systems. It is present normal bodily fluids, elevated pathology (1). In vivo, "LPA" exists as distinct molecular species, each having a single fatty of varying chain length degree unsaturation covalently attached to the glycerol backbone via an acyl, alkyl, or alkenyl link. These species differ affinities for individual LPA receptors [(LPARs), LPA1-6] coupling G proteins (2). However, 18:1 has been continues be most commonly utilized reported studies. remain poorly defined oral biology pathophysiology. Our laboratory addressed this knowledge gap by studying vitro major human salivary [18:1, 18:0, 16:0 (3)] cells (4-7). This includes gingival fibroblasts (GF), which our flow cytometry data from multiple donors found that they express LPA1-5 (6). We have also these are ten-fold pharmacologic levels saliva crevicular fluid obtained patients with moderate-severe periodontitis (8). As potential regulate transcriptional activity had not examined system, study used whole genome microarray analysis test hypothesis 18:1-treated GF would show significant changes gene transcripts relevant their biology, wound-healing, inflammatory responses. was significantly large, complex set genes categories periodontal disease. raw deposited at NCBI's GEO database record GSE57496.