Reappraisal of the expression of mast cell proteases of Mongolian gerbils (Meriones unguiculatus).

摘要: Mast cell proteases in the tongue and jejunum of Mongolian gerbils (Meriones unguiculatus) were examined by enzyme-histochemical methods. Both trypsin-like (tryptase) chymotrypsin-like (chymase) protease activities demonstrated mast cells fresh cryosections. When frozen sections post-fixed various fixatives, those fixed Carnoy's fluid showed strongest enzyme activities. Tryptase chymase paraffin both tissues well preserved when at 4°C for 15 min. However, tissues, especially tongue, drastically reduced longer fixation time higher temperature. Carnoy-fixed (4°C min) treated with heparinase I or chondroitinase ABC before stainings proteases, tryptase lost entirely mostly digestion, slightly organs digestion. In contrast, sites not influenced these pretreatments. These results show that although as contain activities, their stability to fixations is variable among so tissue conditions are crucial preservation. At least some part dependent on proteoglycans present granules.