A medium for the maintenance of Chironomus tentans salivary glands in vitro
作者： Conrad E. Firling , Brian K. Kobilka
DOI: 10.1016/0022-1910(79)90042-8
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摘要: Abstract A synthetic medium based upon the chemical composition of fourth instar Chironomus haemolymph was formulated for in vitro culture tentans salivary glands. Salivary glands maintained up to 4 days appeared morphologically normal. Secretion-free glands, obtained from pilocarpine-treated larvae, accumulated proteinaceous material gland lumen and exhibited a 46% increase total protein after 24 hr medium. Cycloheximide almost totally inhibited accumulation secretion by cultured Glands continued incorporate 14 C-leucine into acid-insoluble 3 H-uridine RNA, but at reduced levels. The incorporation both isotopes completely cycloheximide. Autoradiographic squash preparations pulse-labelled with H-thymidine revealed normal pattern asynchronous chromosomal DNA replication. nucleoli distinct regions which corresponded sites cytochemically demonstrable acidic protein. chromosomes normal, except some regression Balbiani rings. Addition ecdysterone media containing previously resulted puff induction IV-2-B locus.
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