α-Amylase immobilization onto functionalized graphene nanosheets as scaffolds: Its characterization, kinetics and potential applications in starch based industries.
作者： Kritika Singh , Garima Srivastava , Mahe Talat , Onkar Nath Srivastava , Arvind Mohan Kayastha
DOI: 10.1016/J.BBREP.2015.07.002
关键词:
摘要: Abstract α-Amylase is imperative for starch and its deriviatized industries. Functionalized graphene sheets were tailored optimized as scaffold α-amylase immobilization using Response Surface Methodology based on Box–Behnken design, with an overall efficiency of 85.16%. Analysis variance provided adequacy to the mathematical model further studies. Native immobilized functionalized characterized transmission scanning electron microscopy, followed by Fourier transform infrared (FTIR) spectroscopy. Wheat conjugated visually evident micrographs while FTIR spectra showed interplay various chemical interactions bonding, during after immobilization. Optimum pH optimum temperature enzyme though remained unchanged but broader range whereas Km a slight decrease (1.32 mg/mL). It also enhanced thermal storage stability retained 73% residual activity 10 uses. These ensemble properties non-toxic nature graphene, makes it viable be absorbed commercially in processing
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Monika Reiss, Andreas Heibges, Jutta Metzger, Winfried Hartmeier, Determination of BOD-values of starch-containing waste water by a BOD-biosensor1This paper was presented at the fifth World Congress on Biosensors, Berlin, Germany, 3–5 June 1998.1 Biosensors and Bioelectronics. ,vol. 13, pp. 1083- 1090 ,(1998) , 10.1016/S0956-5663(98)00071-2
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
HIDETSUGU FUWA, A NEW METHOD FOR MICRODETERMINATION CF AMYLASE ACTIVITY BY THE USE OF AMYLOSE AS THE SUBSTRATE Journal of Biochemistry. ,vol. 41, pp. 583- 603 ,(1954) , 10.1093/OXFORDJOURNALS.JBCHEM.A126476
Javad Zolgharnein, Ali Shahmoradi, Jahan B. Ghasemi, Comparative study of Box–Behnken, central composite, and Doehlert matrix for multivariate optimization of Pb (II) adsorption onto Robinia tree leaves Journal of Chemometrics. ,vol. 27, pp. 12- 20 ,(2013) , 10.1002/CEM.2487
Dhanya Gangadharan, Swetha Sivaramakrishnan, K. Madhavan Nampoothiri, Rajeev K. Sukumaran, Ashok Pandey, Response surface methodology for the optimization of alpha amylase production by Bacillus amyloliquefaciens Bioresource Technology. ,vol. 99, pp. 4597- 4602 ,(2008) , 10.1016/J.BIORTECH.2007.07.028
Naveen Kumar Mekala, Reeta Rani Singhania, Rajeev K. Sukumaran, Ashok Pandey, Cellulase Production Under Solid-State Fermentation by Trichoderma reesei RUT C30: Statistical Optimization of Process Parameters Applied Biochemistry and Biotechnology. ,vol. 151, pp. 122- 131 ,(2008) , 10.1007/S12010-008-8156-9
Linqiu Cao, Luuk van Langen, Roger A Sheldon, Immobilised enzymes: carrier-bound or carrier-free? Current Opinion in Biotechnology. ,vol. 14, pp. 387- 394 ,(2003) , 10.1016/S0958-1669(03)00096-X
Roger A. Sheldon, Sander van Pelt, Enzyme immobilisation in biocatalysis : Why, what and how Chemical Society Reviews. ,vol. 42, pp. 6223- 6235 ,(2013) , 10.1039/C3CS60075K
Swati B. Jadhav, Rekha S. Singhal, Conjugation of α-amylase with dextran for enhanced stability: process details, kinetics and structural analysis. Carbohydrate Polymers. ,vol. 90, pp. 1811- 1817 ,(2012) , 10.1016/J.CARBPOL.2012.07.078
Shakeel Ahmed Ansari, Qayyum Husain, Potential applications of enzymes immobilized on/in nano materials: A review Biotechnology Advances. ,vol. 30, pp. 512- 523 ,(2012) , 10.1016/J.BIOTECHADV.2011.09.005