Mucin-type core 1 glycans regulate the localization of neuromuscular junctions and establishment of muscle cell architecture in Drosophila.
作者： Kazuyoshi Itoh , Yoshihiro Akimoto , Takashi J. Fuwa , Chikara Sato , Akira Komatsu
DOI: 10.1016/J.YDBIO.2016.01.032
关键词:
摘要: T antigen (Galβ1-3GalNAcα1-Ser/Thr), a core 1 mucin-type O-glycan structure, is synthesized by Drosophila β1,3-galactosyltrasferase (dC1GalT1) and expressed in various tissues. We previously reported that dC1GalT1 synthesizes hemocytes, lymph glands, the central nervous system (CNS) mutant larvae display decreased numbers of circulating hemocytes excessive differentiation hematopoietic stem cells glands. have also been shown to morphological defects CNS. However, functions other tissues remain largely unknown. In this study, we found glycans contributed localization neuromuscular junction (NMJ) boutons. larvae, NMJs were ectopically formed cleft between muscles 6 7 connected with these two muscles. antigen, which was at NMJs. addition, determined function O-glycans muscle cells. muscles, myofibers basement membranes disorganized. Moreover, ultrastructural accumulation large endosome-like structures within both NMJ boutons observed mutants. Taken together, results demonstrated involved boutons, synaptogenesis NMJs, establishment cell architecture, endocytosis.
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