Phenotype‐based clustering of glycosylation‐related genes by RNA i‐mediated gene silencing

摘要: Glycan structures are synthesized by a series of reactions conducted glycosylation-related (GR) proteins such as glycosyltransferases, glycan-modifying enzymes, and nucleotide-sugar transporters. For example, the common core region glycosaminoglycans (GAGs) is sequentially peptide-O-xylosyltransferase, β1,4-galactosyltransferase I, β1,3-galactosyltransferase II, β1,3-glucuronyltransferase. This raises possibility that functional impairment GR involved in synthesis same glycan might result phenotypic abnormality. To examine this possibility, comprehensive silencing genes encoding proteoglycan was Drosophila. Drosophila candidate (125) were classified into five groups for GAGs, N-linked, O-linked, Notch-related, unknown glycans. Spatiotemporally regulated caused range malformed phenotypes fell three types: extra veins, thick depigmentation. The clustered reflected biosynthetic pathways Fringe-dependent on Notch, glycans placed at or near nonreducing ends (herein termed terminal domains glycans). Based clustering, CG33145 predicted to be formation domains. Our further analysis showed exhibited galactosyltransferase activity N-linked Phenotypic therefore, has potential prediction novel genes.