Framework for maximizing throughput in preparative liquid chromatography

摘要: Abstract This paper addresses the questions: (a) Should one use volume or concentration overloading both to achieve maximum throughput in preparative liquid chromatography? (b) Is plate height concept valid for overloaded columns? (c) What are main factors limiting throughput? (d) column lengths, L, and particle diameters, dp, provide We conclude that will always greatest but injected sample may be a substantial fraction of eluted peak, peak widths increase as square root distance migrated under overload can used optimisation calculations, major restriction on is minimum number theoretical plates, N*, required adequate resolution solutes being isolated, achieved specific ratio dp2/L determined by N* other operating parameters. In practice values between 2000 50 we likely columns 200–2000 mm long packed with 10–60 μm diameter particles giving throughputs 0.1 20 g/h per cm2 cross section at capacity 4.