Large sample volumes in preparative chromatography
作者： Robert A. Barford , Robert McGraw , H.L. Rothbart
DOI: 10.1016/S0021-9673(00)95618-8
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摘要: Abstract Equations are developed which relate output profiles resulting from large sample input volumes to the well-known expressions for elution chromatography. The such that intermediate usual Gaussian curves very small volumes, and frontal described by an integral equation. Simple curve height at maximum width as a function of volume presented. This information is used apparent plate variation in observed resolution ( R ′ s ) volume. It demonstrated 1/ = (1/ + v /Δ V ). equations tested reversed-phase high-performance liquid chromatography preparative with silica stationary phase.
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