Functionalized Folic Acid-Conjugated Amphiphilic Alternating Copolymer Actively Targets 3D Multicellular Tumour Spheroids and Delivers the Hydrophobic Drug to the Inner Core.

摘要: Engineering of a “smart” drug delivery system to specifically target tumour cells has been at the forefront cancer research, having engineered for safer, more efficient and effective use chemotherapy treatment cancer. However, selective targeting choosing right surface biomarker are critical targeted work. Currently, available systems two-dimensional monolayer test efficacy system, but designing be specific in vivo penetrate inner core remains major design challenge. These challenges can overcome by using study model that integrates three-dimensional aspect culture system. Here, we tested functionalized folic acid-conjugated amphiphilic alternating copolymer poly(styrene-alt-maleic anhydride) (FA-DABA-SMA) via biodegradable linker 2,4-diaminobutyric acid (DABA) avascular human pancreatic breast spheroids culture. The was quantitatively analyzed its hydrophobic encapsulation efficiency three different chemical structures with molecular weights. Their release profiles properties various concentrations pH environments were also characterized. Using anticancer curcumin two standard clinical chemotherapeutic drugs, paclitaxel 5-fluorouracil, ability FA-DABA-SMA nanoparticles encapsulate differently sized drugs deliver them kill WST-1 cell proliferation assay. findings this revealed shows unique as an active tumor-targeting internalize chemotherapeutics killing cells. novelty is first demonstrate encapsulated effectively penetrating reducing their volumes dose- time-dependent manner.