Ribonucleic acid-permeable mutant of Escherichia coli

摘要: Abstract An RNA-permeable mutant was isolated from a tryptophan amber auxotrophic strain of Escherichia coli after mutagenesis with N -methyl- N' -nitro- -nitrosoguanidine. The rationale the isolation based on suppression an mutation. A selected, which could grow in minimal medium supplemented transfer RNA prepared suI -carrying but not su − strain. This incorporated 3 H-labeled bulk into cells at rate 40 times higher than did parent level requirement, susceptibility to lytic action lysozyme and RNase activity culture differ those ribosomal equally as well it incorporation competitively inhibited by any species cold RNA. However, fate rRNA resulted degradation yield acid-soluble fragments whereas tRNA remained intact cell. From these results, concluded that selected basis actually incorporate molecules without loss their functions.