Detection of Porcine Endogenous Retrovirus (PERV) Viremia in Diseased Versus Healthy US Pigs by Qualitative and Quantitative Real‐Time RT‐PCR

摘要: Previous studies have linked levels of porcine endogenous retroviruses (PERV) with poor health and disease in pigs. To determine the expression PERVs their potential association expression, real-time reverse transcriptase (RT) PCR assays were used to assess PERV-ABC, PERV-C PERV-A/C three commercial swine operations United States. Pigs (n = 204) aged 3-25 weeks screened, all 369 serum samples collected found be positive for PERV-ABC RNA as expected. detected 24.1% (89/369) 18.7% (69/369) samples, respectively. When divided into age groups, was identified 20.0% (43/215) nursery pig (3-9 age) compared 16.9% (26/154) finisher (12-25 age). On two farms, from healthy pigs 60) pen-mates various clinical conditions including diarrhoea, wasting respiratory 60). Overall, 25% (15/60) clinically affected RNA, whereas pigs, only 8.3% (5/60) (P 0.026). It possible identify same on more than one consecutive bleeding, indicating variable length viremia. The results indicate that there is an increased incidence viremia diseased can over time selected within production systems