Recovery of hair cell function after damage induced by gentamicin in organ culture of rat vestibular maculae.

摘要: Here, we report the functional and morphological evidence of hair cell recovery after damages induced by gentamicin (GM) in cultured explants rat vestibular maculae. We evaluated mechano-electrical transduction (MET) function cells, measuring Ca(2+) responses with fura-2 when bundles were stimulated. After MET testing, observed high resolution scanning electron microscopy, or fluorescence microscopy staining phalloidin-FITC (fluorescent isothiocyanate). In control culture, number on gradually decreased, percentage showing decreased disappeared 17 days culture. Following GM (1-2 mM) treatment, most eliminated initially, but increased during Short bundle-like structures emerged areas where had been completely lost. Consistent observations, they recovered to a peak 13-17 treatment even at more Furthermore, cells accumulated FM1-43, dye permeable through channel, treatment. Application steroid hormone activity, enhanced investigated Ki-67 immunoreactivity detect proliferation TUNEL apoptotic death. was negative however positive positivity dose dependent. Therefore, this activity not owing likely self-repair bundle.