An ATP-Activated Channel Is Involved in Mitogenic Stimulation of Human T Lymphocytes

摘要: We investigated the effect of pharmacologic modulation ATP receptor on intracellular ion changes and proliferative response human peripheral blood lymphocytes (PBLs) purified T lymphocytes. Extracellular (ATPe) triggered in these cells an increase cytoplasmic Ca2+ concentration ([Ca2+]i) plasma membrane depolarization. Whereas both release from stores influx across were detected whole PBL population, only was observed cells. In presence near physiologic extracellular Na+ concentrations (125 mmol/L), permeability through ATPe-gated channel very low, suggesting a higher selectivity for monovalent over divalent cations. The selective P2Z agonist benzoylbenzoic (BzATP) increased [Ca2+]i but not absence also caused covalent blocker oxidized (oATP), inhibitor P2X receptors, prevented depolarization, had no stores. Stimulation with ATPe alone significant effects 3H-thymidine incorporation. On contrary, or BzATP synergistic DNA synthesis stimulated by T-cell mitogens such as phytohemagglutinin, anti-CD3 monoclonal antibody, allogenic PBLs (mixed lymphocyte cultures). Treatment oATP inhibited mitogenic stimulation receptor-directed agents combined application ionophore ionomycin phorbol myristate acetate. Interleukin-2 partially relieved inhibition oATP. These results suggest that express gated is involved stimulation.