Comparison of embryonic developmental competence of mouse oocytes grown with and without serum.

摘要: The first objective of this study was to determine whether oocyte growth in serum-free medium affects the solubility zona pellucida α-chymotrypsin digestion, which is an index “hardening” and reflects potential penetrability by sperm. Oocyte-granulosa cell complexes were isolated from preantral follicles 12-day-old mice cultured for 10 days containing 5% fetal bovine serum (FBS) or medium. zonae pellucidae oocytes grown four times as hard freshly germinal vesicle (GV)-stage vivo vitro FBS-containing hardening prevented addition fetuin. second compare competence undergo embryogenesis that grew vs. Approximately 70% underwent maturation regardless contained FBS. Of mature ova FBS, 53% cleaved two-cell stage after insemination compared with only 6% Addition fetuin used increased frequency cleavage stage. embryos derived medium, completed blastocyst transition. In contrast, about 40% (P < 0.01) transition supplemented Nevertheless, 12% two- four-cell-stage matured developed live young transfer oviducts foster mothers. These results suggest serum-borne factors may function during promote events leading successful fertilization embryogenesis.