Pulsed-Field Map of Xq13 in the Region of the Human X Inactivation Center

摘要: We have used human/mouse hybrid cell lines to derive a pulsed-field map of the Xq13 region human X chromosome, in vicinity inactivation center (XIC). mapped nine loci within two separate clusters (I and II). Cluster I contains three (DXS227, XIST, DXS128) linked 1700 kb. This cluster also includes breakpoint translocated X;14 chromosome define proximal border XIC region. II covers an additional 1800 kb physically links six (DXS56, DXS171, DXS325, DXS347, DXS356, DXS441) located between genes for Menkes disease (MNK) PGK1. Maps derived from active (Xa) or inactive (Xi) chromosomes differed, presumably due methylation differences Xa Xi. provides basis examining organization Xq13.2-q13.3 region, around XIC, will assist further cloning expressed sequences this