Biochemical research on oogenesis. Transfer RNA is fully charged in the 42-S storage particles of Xenopus laevis oocytes.
作者： Maurice WEGNEZ , Herman DENIS
DOI: 10.1111/J.1432-1033.1979.TB13161.X
关键词:
摘要: 1. Transfer RNA makes up 30-40% of total in previtellogenic oocytes Xenopus laevis. The bulk tRNA is associated with 5-S and two proteins a high-molecular-weight complex sedimenting at 42S. 2. We show here that all kinds are present the 42-S particles them sediment coincidently. Particle fully charged vivo. During purification becomes partially uncharged. When purified incubated vitro amino acids ATP charging reaction occurs without disruption nucleoprotein complex. Many aminoacyl-tRNA synthetases can be shown to co-sediment particles. conclude complete aminoacylation within storage results from activity particle-bound synthetases.
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