Evolution, expression, and chromosomal location of a novel receptor tyrosine kinase gene, eph.

摘要: Partial sequence analysis of the genomic eph locus revealed that splicing points kinase domain-encoding exons were completely distinct from those other protein tyrosine members reported, suggesting this is earliest evolutionary split within family. In Northern (RNA) blot analysis, gene was expressed in liver, lung, kidney, and testis rat, screening 25 human cancers various cell types showed preferential expression cells epithelial origin. Overexpression mRNA found a hepatoma lung cancer without amplification. Comparison cDNA sequences derived normal liver overproduces demonstrated two them identical throughout transmembrane to carboxy-terminal portions. Southern DNAs human-mouse hybrid clones with an probe present on chromosome 7.