Inhibition of biofilm formation in Mycobacterium smegmatis by Parinari curatellifolia leaf extracts

摘要: Tuberculosis (TB) is a serious public health problem worldwide. Mycobacterium tuberculosis (M. tuberculosis) grows as drug tolerant pellicles. Agents that inhibit biofilm formation in M. have the potential to reduce disease treatment period and improve quality of chemotherapy. Parinari curatellifolia (P. curatellifolia) leaf extracts are claimed treat symptoms similar ethnomedicinal practices. smegmatis smegmatis) surrogate organism used antimycobacterial discovery assays. In this study, effect P. on growth was investigated order determine basis its use traditional medicinal use. Phytochemicals from leaves were prepared using mixture 50% dichloromethane (DCM): methanol by serial exhaustive extraction different solvents decreasing polarity. The following order, hexane > ethyl acetate acetone >ethanol water. micro-broth dilution method an susceptibility test screen for extract effectively inhibited formation. Biofilm quantification performed staining biofilms with crystal violet determining amount stain spectrophotometer. addition, effects combining most active kanamycin also investigated. minimum inhibitory concentrations (MIC) found be 6.2 μg/ml extract, 12.5 μg/ml both ethanol total 50 μg/ml extracts. water only smegmatis. Combining enhanced terms inhibition contain phytochemicals anti-biofilm compounds.