Potency of endotoxin from bicarbonate dialysate compared with endotoxins from Escherichia coli and Shigella flexneri.

摘要: Endotoxin is a potent activator of the complement system and other host immunoregulators, including cytokines, tumor necrosis factor alpha, interleukin-1 beta, interleukin-6. In this study, potency an endotoxin from bicarbonate dialysate was compared with endotoxins two enteric microorganisms, Shigella flexneri Escherichia coli. concentrations were standardized for three by use Limulus amebocyte lysate turbidimetric assay. assessed comparative plasma interleukin-6 after in vitro whole-blood challenge each type endotoxin. Blood collected 10 hemodialysis patients spiked 0.1, 1, ng/mL E. coli 1 After incubation, separated frozen at -70 degrees C until assayed cytokine concentrations. Dialysate found to be 100 times less than endotoxins. It concluded that there are significant differences different strains bacteria these should noted when designing or evaluating studies on clinical effects settings.