Calbindin D28K Gene Expression in Neurodegenerative Diseases

摘要: Publisher Summary This chapter focuses on calbindin D 28K gene expression in aging and neurodegenerative diseases. The study described the has permitted an analysis of role that changes may play etiology pathogenesis neurodegeneration brain, such as Huntington's, Parkinson's, Alzheimer's A method molecular cloning resulted isolation a cloned cDNA via direct immunological screening mouse cerebellar λgt11 bacterial library. could then be radiolabeled directly utilized probe for detection mRNA (slot-blot Northern blot analyses) or converted to cRNA transcripts detect at cellular level by situ hybridization. Slot-blot hybridization analyses revealed each disease entity, brain area is particularly affected used hallmark neuropathological exhibits significant reduction expression.