Corticosterone regulates calbindin-D28k mRNA and protein levels in rat hippocampus.
作者： A M Iacopino , S Christakos
DOI: 10.1016/S0021-9258(18)86924-1
关键词:
摘要: Corticosterone was administered to normal and bilaterally adrenalectomized rats (250-300 g), hormonal regulation of brain calbindin-D28k (CaBP28k) levels investigated by radioimmunoassay for CaBP28k protein slot Northern blot analyses mRNA. The specificity the changes observed in mRNA tested reprobing blots with calmodulin B-actin cDNAs. Rats were either adrenalectomized, treated corticosterone, intact, or intact corticosterone. Chronic corticosterone administration (subcutaneous injection 7 days, 10 mg/day) significantly increased immunoreactivity (43%) (125%) hippocampus. Adrenalectomy (animals killed days after adrenalectomy) produced a significant decrease hippocampal (85%) (80%) compared controls. Immunocytochemical analysis tissue sections inducated marked depletion dentate gyrus hippocampus 2 weeks adrenalectomy. When (10 mg/day days), restored No kidney, cerebellum, striatum, cerebral cortex noted when controls, indicating effect on These studies present first evidence regulator gene expression brain.
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