Approaches for identifying multiple-SNP haplotype blocks for use in human identification.

摘要: Single nucleotide polymorphism (SNP) discrimination effectiveness is low due to the bi-allelic nature of SNPs, and large numbers loci must be analyzed for human identification in forensic casework. To resolve these issues, authors support use multiple SNP haplotypes that will generate many based on combination alleles. First, 27 regions were selected from JSNP database (http://snp.ims.u-tokyo.ac.jp) according following criteria: (1) 3 or more within 100 bp; (2) on-intron out-of-gene location; (3) frequency than 40% each allele. PCR amplification high-resolution melting curve (HRM) analysis then carried out all determine variation each. HRM indicated 7 (1q25, 1q42.2, 3p24, 10p13, 11p15.1, 14q12–q13, 20q12) containing had 2 haplotypes. The frequencies region observed via direct sequencing individuals. Not only haplotyping increases individual but also shorter common short tandem repeat analysis, representing a further advantage fragmented DNA samples typing.