Perspective and limitations of cryo-electron microscopy. From model systems to biological specimens.
作者： P. M. Frederik , M. C. A. Stuart , P. H. H. Bomans , W. M. Busing , K. N. J. Burger
DOI: 10.1111/J.1365-2818.1991.TB03088.X
关键词:
摘要: SUMMARY We investigated the possibility of vitrifying temperature-sensitive lipid phases as well (small) biological specimens. From a suspension unilamellar vesicles, prepared from dipalmitoyl-phosphatidylcholine (DPPC), thin aqueous films were formed at various temperatures. With cryo-electron microscopy vesicles found to be smooth, rippled and faceted or only, depending on temperature thin-film formation (318, 312 296 K respectively). The morphology electron diffraction patterns indicate that membranes can by physically fixed vitrification in their high-temperature configuration studied low microscopy. This finding suggests it may also possible preserve, original state, more complex membrane systems living organisms initiating rapid-cooling physiological temperature. was explored specimen grids with (human) blood platelets adhering collagen fibres. Low-temperature observation an acceleration voltage 120 kV revealed subcellular details. More details observed when using higher accelerating voltages (200 300 kV) beam. results presented this paper illustrate great potential study dynamics, both relatively simple model systems.
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