Assembly and use of a broadly applicable neural cDNA microarray.

摘要: cDNA microarrays provide an efficient method to analyze gene expression patterns in thousands of genes parallel. In some cases, large unfocused collections cDNAs have been used hybridization studies, others small logically defined tissue specific arrays used. Here we describe the bioinformatic selection 1152 named human specifically designed for neuroscience applications, arrayed on nylon membranes at high density, were chosen which represent all major cellular types brain including; neurons, astrocytes, microglia, and oligodendrocytes. Gene families include cell type markers, ion-channels, transporters, receptors, adhesion molecules among many others. These with region samples from determine mRNA profiles each region. Used 33 -P labeled complex probes, this is a low cost, highly sensitive approach investigator focus interest where limiting amounts RNA are This selected set brain-relevant should be widely useful analysis tissues as well neural lines.