In silico gene selection strategy for custom microarray design.

摘要: Microarray technology has become an important tool for studying large-scale gene expression a diversity of biological applications. However, there are number experimental settings which commercial arrays either unsuitable or unavailable despite the existence sequence information. With increasing availability custom array manufacturing services, it is now feasible to design high-density any organism having data. have been relatively few reports discussing selection, first step in design. Here we propose silico strategy microarray selection that applicable wide range organisms, based on utilizing public domain information interrogate existing data and identify set homologous genes interest. We demonstrate utility this approach by applying candidate Xenopus laevis microarray. A significant finding study 3%-4% expressed tags (ESTs) orientation contrary indicated database entry (http://mssaha.people.wm.edu/suppMSS.html).