Adenylate Cyclase in Intestinal Crypt and Villus Cells: Stimulation by Cholera Enterotoxin and Prostaglandin E1
作者： Charles J. Schwartz , Daniel V. Kimberg , Patricia Ware
DOI: 10.1016/S0016-5085(75)80053-9
关键词:
摘要: The secretory responses to cholera enterotoxin and prostaglandin E1 (PGE1) are dependent upon elevation of the intracellular levels cyclic AMP. Although several previous reports have suggested that intestinal secretion due PGE1 may be confined crypt cells, this matter has been incompletely resolved. These studies were undertaken define activity adenylate cyclase in villus cells from rabbit rat intestine, determine influence on activity. Mucosal fractions prepared ileum with a planing device, distal small intestine by vibration technique. In both species base line was greater than cells. After exposure vivo, enhanced all ileum, response most marked Furthermore, membranes responsive vitro addition PGE1. results these indicate contain one important components required for AMPmediated response, namely, PGE1-sensitive Since an increased level AMP alone not sufficient evoke do clarify extent which each major cell types participate AMP-mediated secretion.
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