Higher levels of thymidylate synthase gene expression are observed in pulmonary as compared with hepatic metastases of colorectal adenocarcinoma
作者： R Gorlick , R Metzger , K D Danenberg , D Salonga , J S Miles
DOI: 10.1200/JCO.1998.16.4.1465
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摘要: PURPOSEIt has been observed previously that the pulmonary metastases of colorectal adenocarcinoma are less responsive to therapy with fluorouracil (FUra) as compared other sites metastasis (liver, local). To investigate basis this chemoresistance, levels thymidylate synthase (TS) mRNA and protein were measured, TS expression shown be predictive response in cancer.MATERIALS AND METHODSTumors obtained from 19 patients metastatic cancer (12 hepatic seven pulmonary). was measured by quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) Western blotting. The presence amplification assessed Southern Levels p53 determined using immunohistochemistry.RESULTSTS significantly higher (mean TS/beta-actin ratio, 19.7; n = 7) (mea...
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